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Related Experiment Video

Updated: Jun 25, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
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Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

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Nanobiotechnology: soft lithography.

Elisa Mele1, Dario Pisignano

  • 1National Nanotechnology Laboratory of Istituto Nazionale di Fisica della Materia-Consiglio Nazionale delle Ricerche, Distretto Tecnologico ISUFI, Università degli Studi di Lecce, via Arnesano, Lecce, I-73100, Italy.

Progress in Molecular and Subcellular Biology
|February 10, 2009
PubMed
Summary
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Soft lithography techniques, including microcontact printing, are revolutionizing nanobiotechnology. These methods enable precise manipulation of biological materials for applications like protein arrays and microfluidic devices.

Area of Science:

  • Nanobiotechnology
  • Biotechnology
  • Materials Science

Background:

  • Nanobiotechnology integrates nanotechnology and biology, leveraging biomolecules as nanosystems and nano-devices for biological studies.
  • Advancements in micro- and sub-micrometre fabrication have led to the development of soft lithography.
  • Soft lithography offers non-photolithographic patterning techniques crucial for biological applications.

Purpose of the Study:

  • To examine the biological applications of various soft lithographic techniques.
  • To highlight the general features of soft lithography and commonly used materials.
  • To present specific methods like microcontact printing and microfluidic lithography for biological materials.

Main Methods:

  • Soft lithography techniques, including microcontact printing (μCP) and microfluidic lithography.

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Last Updated: Jun 25, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
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Published on: June 12, 2018

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior
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  • Patterning of selective protein and cell attachment.
  • Fabrication of microfluidic circuits and 3D scaffolds.

    • Main Results:

    • Soft lithography has become integral to nanobiotechnology.
    • Techniques enable selective attachment of proteins and cells.
    • Applications include protein and DNA microarrays and microfluidic analytical devices.

    Conclusions:

    • Soft lithography is a powerful tool in nanobiotechnology for biological applications.
    • Microcontact printing and microfluidic lithography are key techniques presented.
    • The study showcases the potential of these methods in creating advanced biological tools and devices.