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Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System
Published on: March 14, 2025
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Microbial Omics
Ahmet Arıhan Erözden1,2, Nalan Tavşanlı1,2, Mahmut Çalışkan1
1Biotechnology Division, Department of Biology, Faculty of Science, Istanbul University,Vezneciler, Istanbul, Türkiye.
Progress in Molecular and Subcellular Biology
|March 26, 2026
Summary
Omics technologies offer powerful tools for microbiology research. This chapter guides researchers on using genomics, transcriptomics, proteomics, metabolomics, and meta-omics for microbial ecosystem analysis.
Area of Science:
- Microbiology
- Molecular Biology
- Bioinformatics
Background:
- Omics technologies have transformed scientific research.
- Their application in microbiology offers novel insights into microbial life and community interactions.
- Understanding microbial ecosystems requires multi-level molecular investigation.
Purpose of the Study:
- To provide a comprehensive overview of single-organism and meta-omics techniques in microbiology.
- To detail the experimental and bioinformatic workflows for various omics approaches.
- To serve as a practical guide for researchers studying microbial structure, function, and interactions.
Main Methods:
- Genomics, transcriptomics, proteomics, metabolomics for individual organisms.
- Metagenomics, metatranscriptomics, metaproteomics, meta-metabolomics for microbial communities.
- Integrative multi-omics strategies for holistic ecosystem analysis.
Main Results:
- Detailed descriptions of key omics methods, including their features and applications.
- Outlines of experimental and bioinformatic pipelines for each technique.
- Discussion of commonly used computational tools for omics data analysis.
Conclusions:
- Omics technologies provide a powerful toolkit for microbial research.
- Multi-omics approaches are essential for a comprehensive understanding of microbial ecosystems.
- This chapter equips researchers with practical knowledge for exploring microbial life at multiple molecular levels.
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