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Updated: Jun 25, 2026

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
The anti-condyloma acuminatum effects of interferon-inducible protein 10 in vitro
Huijuan Li1, Tao Yi, Shuyun Zhao
1Department of Gynecology and Obstetrics, West China Second University Hospital, and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Background:
Interferon-inducible protein 10 (IP-10) has been reported to show an effective antiviral and antineoplastic role in various murine models.
Aim:
To investigate the anti-condyloma acuminatum effect and human papillomavirus (HPV)-inhibiting efficacy of a recombinant plasmid encoding IP-10 in vitro.
Methods:
A recombinant plasmid DNA carrying IP-10 cDNA was constructed. Condyloma acuminatum tissue particles were transfected with IP-10 and examined for apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry techniques. Relative real-time polymerase chain reaction (PCR) was used to validate the HPV inhibited level of the treatment groups. Immunohistochemistry was used to determine the IP-10 expression on condyloma acuminatum tissues.
Results:
Transfected IP-10 was expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful apoptosis was observed in condyloma acuminatum tissues transfected with IP-10. In addition, HPV expression was lower in IP-10-treated tissues than in control tissues.
Conclusions:
These observations suggest that IP-10 has strong anti-condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and therefore may be a novel and potentially effective therapy for condyloma acuminatum.
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