Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Validation of a novel computer-assisted sperm analysis (CASA) system using multitarget-tracking algorithms.

Mathew James Tomlinson¹, Karen Pooley, Tracey Simpson

¹Fertility Unit, Nottingham University Hospital, Nottingham, United Kingdom. mathew.tomlinson@nuh.nhs.uk

Fertility and Sterility

|February 10, 2009

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A conversational multi-agent AI system for automated plant phenotyping.

Nature communications·2026

Same author

The problem with disclosing IVF status: Normative parental timelines and the gendered support needs of involuntary childless heterosexual couples undergoing in vitro fertilisation.

Journal of health psychology·2025

Same author

Data management challenges for artificial intelligence in plant and agricultural research.

F1000Research·2023

Same author

Meeting sustainable development goals via robotics and autonomous systems.

Nature communications·2022

Same author

Sperm toxicity testing on lubricant gels: should we be recommending 'fertility-friendly' specialist products?

Human fertility (Cambridge, England)·2022

Same author

Temporal refinement of 3D CNN semantic segmentations on 4D time-series of undersampled tomograms using hidden Markov models.

Scientific reports·2021

A new computer-assisted sperm analysis (CASA) system shows high accuracy and repeatability for sperm concentration and motility measurements. This novel CASA system is as reliable as traditional manual methods for semen analysis.

Area of Science:

Reproductive Medicine
Andrology
Medical Technology

Background:

Accurate semen analysis is crucial for male fertility assessment.
Traditional manual methods for sperm analysis can be labor-intensive and subjective.
Computer-assisted sperm analysis (CASA) offers potential for objective and efficient evaluation.

Purpose of the Study:

To evaluate the accuracy and precision of a novel computer-assisted sperm analysis (CASA) system.
To compare the performance of the new CASA system against established manual methods for sperm analysis.

Main Methods:

A prospective study was conducted comparing CASA and manual methods.
Measurements included sperm concentration and motility using latex beads and sperm samples.
Accuracy was assessed by comparing to target values, and precision by coefficients of variation and intraclass correlation coefficients.

More Related Videos

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

Main Results:

The novel CASA system demonstrated high accuracy and repeatability for sperm concentration measurements.
Sperm counts obtained via CASA and manual methods were found to be interchangeable.
While CASA and manual motility measurements were highly correlated, manual estimates for motile sperm were consistently higher.

Conclusions:

The novel CASA system provides reliable semen quality measurements for sperm concentration.
The system's motility measurements are comparable to manual methods, though direct interchangeability requires further investigation.
This CASA system offers a reliable alternative to current manual techniques for semen analysis.