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Orthotopic Hind Limb Transplantation in the Mouse
Published on: February 12, 2016
Intragraft chimerism following composite tissue allograft
Keiichi Muramatsu1, Ryutaro Kuriyama, Toshihiko Taguchi
1Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Japan. muramatu@yamaguchi-u.ac.jp
The Journal of Surgical Research
|February 10, 2009
Summary
Recipient cells repopulate extremity allografts, influencing acceptance and rejection. This cell traffic, known as intragraft chimerism, is crucial for understanding transplant outcomes in hand transplants.
Area of Science:
- Transplantation immunology
- Cell biology
- Regenerative medicine
Background:
- Hand transplants are increasing, raising interest in graft acceptance mechanisms.
- Intragraft chimerism (recipient cell traffic into the graft) is implicated in graft acceptance and rejection.
- Limited knowledge exists on cell migration patterns in extremity allografts.
Purpose of the Study:
- To review experimental studies on intragraft chimerism in extremity allografts.
- To elucidate the role of recipient cell migration in allograft acceptance.
- To identify tissues involved in cell traffic within extremity transplants.
Main Methods:
- Immunohistochemistry
- Karyotyping
- Fluorescent in situ hybridization (FISH)
- Polymerase chain reaction (PCR)
- Transgenic animal models
Main Results:
- Recipient-derived cells were found to repopulate grafted skin, bone, bone marrow, and endothelial cells.
- Muscle, periosteum, and cartilage tissues largely retained donor cell origin.
- Demonstrates a differential repopulation pattern across various tissue types in extremity allografts.
Conclusions:
- Intragraft chimerism is a significant factor in extremity allograft acceptance.
- Understanding cell migration patterns can inform strategies to improve transplant outcomes.
- Tissue-specific cellular dynamics influence the success of allografts.
