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Updated: Jun 25, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Sandwich-type electrochemiluminescence immunosensor based on N-(aminobutyl)-N-ethylisoluminol labeling and gold
Dayong Tian1, Chunfeng Duan, Wei Wang
1Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Abstract:
A novel electrochemiluminescence (ECL) sandwich-type immunosensor for human immunoglobulin G (hIgG) on a gold nanoparticle modified electrode was developed by using N-(aminobutyl)-N-ethylisoluminol (ABEI) labeling. The primary antibody, goat-anti-human IgG was first immobilized on a gold nanoparticle modified electrode, then the antigen (human IgG) and the ABEI-labeled second antibody was conjugated successively to form a sandwich-type immunocomplex. ECL was carried out with a double-step potential in carbonate buffer solution (CBS) containing 1.5mM H(2)O(2). The ECL intensity increased linearly with the concentration of hIgG over the range 5.0-100 ng/mL. The limit of detection was 1.68 ng/mL (S/N=3). The relative standard deviation was 3.79% at 60 ng/mL (n=9). The present immunosensor is simple and sensitive. It has been successfully applied to the detection of hIgG in human serums.
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