Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Packaging00:58

DNA Packaging

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stress unmasks impaired endocrine coordination of glucose metabolism in dystrophin deficiency.

bioRxiv : the preprint server for biology·2026
Same author

Intra-articular Methotrexate-Loaded Microsponge as an Adjuvant Strategy for Rheumatoid Arthritis: Localized Treatment with a Systemic Impact.

ACS biomaterials science & engineering·2026
Same author

The Remarkable Properties of Oil-in-Water Zein Protein Microcapsules.

Molecules (Basel, Switzerland)·2026
Same author

Trafficking Glycogen Nanoparticles through Lymph Node Tissues for the Delivery of Small and Large Bioactive Molecules.

ACS nano·2025
Same author

Antimicrobial peptides boosted by ultrasound.

Nature biomedical engineering·2025
Same author

Ultrasound-Driven Coassembly of Anticancer Drugs into Carrier-Free Particles.

ACS nano·2025

Related Experiment Video

Updated: Jun 25, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Assembly and functionalization of DNA-polymer microcapsules.

Francesca Cavalieri1, Almar Postma, Lillian Lee

  • 1Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.

ACS Nano
|February 12, 2009
PubMed
Summary

Researchers developed novel DNA-polymer microcapsules using poly(N-isopropylacrylamide) (PNIPAM) and DNA. These versatile microcapsules show potential for controlled therapeutic delivery due to their tunable properties and functionalization capabilities.

More Related Videos

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

Related Experiment Videos

Last Updated: Jun 25, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Nanotechnology

Background:

  • Stimuli-responsive polymers like poly(N-isopropylacrylamide) (PNIPAM) offer tunable properties for advanced material applications.
  • DNA nanotechnology provides precise control over molecular assembly and functionalization.
  • The development of hybrid DNA-polymer systems is crucial for creating advanced drug delivery vehicles.

Purpose of the Study:

  • To synthesize and characterize DNA-grafted PNIPAM micelles.
  • To assemble these micelles into multilayered thin films and DNA-PNIPAM microcapsules.
  • To functionalize the microcapsules with poly(ethylene glycol) (PEG) for enhanced properties and therapeutic applications.

Main Methods:

  • Sequential deposition of DNA-grafted PNIPAM micelles (PNIPAM-A(30) or PNIPAM-T(30)) to form multilayered films.
  • Alternate deposition of PNIPAM-A(30) and PNIPAM-T(30) onto silica particles to create microcapsules, followed by core removal.
  • Utilizing alkyne 'click' chemistry on the PNIPAM micelle core for covalent coupling of azide-bearing PEG.

Main Results:

  • Successful synthesis and characterization of DNA-PNIPAM micelles and their assembly into microcapsules.
  • Observed 30-50% shrinkage of microcapsules upon silica core removal.
  • Demonstrated reduced permeability of DNA-PNIPAM microcapsules to dextran compared to DNA-only microcapsules.
  • Achieved covalent coupling of PEG to the microcapsules via 'click' chemistry.

Conclusions:

  • The developed DNA-PNIPAM microcapsules are multifunctional and versatile, combining hydrophobic and reactive nanodomains with DNA degradability.
  • These hybrid microcapsules exhibit reduced permeability and can be functionalized with PEG, making them promising for controlled therapeutic delivery.
  • The system offers a robust platform for advanced applications in nanomedicine and drug delivery.