Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Enzyme nanorings.

Tsui-Fen Chou1, Christopher So, Brian R White

  • 1Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.

ACS Nano
|February 12, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T-cell Receptor (TCR) Targeting with Multivalent T-cell Engagers.

bioRxiv : the preprint server for biology·2026
Same author

Time-Resolved Proteomic Analysis in Zebrafish Using Bioorthogonal Noncanonical Amino Acid Tagging.

Journal of proteome research·2026
Same author

Global analysis of protein degradation reveals instability of diverse regulators in <i>Escherichia coli</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Surgical Complications in Hirschsprung Disease and the Impact of Botulinum Toxin Injection on Hirschsprung-Associated Enterocolitis.

Journal of clinical medicine·2026
Same author

Regulation of mitophagy by Fis1 and Fascin1-organized actin.

Current biology : CB·2026
Same author

Male-biased Yap1-Cd276/B7-H3 axis for immune evasion in medulloblastoma.

Cancer cell·2026
Same journal

Vertically Stacked Indium Gallium Zinc Oxide-Based Three-Dimensional Integrated Circuits.

ACS nano·2026
Same journal

Tunable Nanoparticle Thin-Film Reveals Distance Dependence of Auger-Mediated Radiation Enhancement in Diffuse Midline Glioma.

ACS nano·2026
Same journal

G-Quadruplex Network Engineering in Ionogels: Realizing Robust Biosensing Interfaces for Plant Electrophysiology.

ACS nano·2026
Same journal

Announcing the 2026 <i>ACS Nano</i> Lectureship and <i>ACS Nano</i> Impact Award Laureates.

ACS nano·2026
Same journal

Ultrafast Self-Assembly of Zeolitic Imidazolate Framework-8 Enables Antibody Orientation for Ultrasensitive Lateral Flow Immunoassays.

ACS nano·2026
Same journal

Interfacial Salt Engineering with Alkali and Ammonium Additives for Stable Pure-Blue Perovskite Light-Emitting Diodes and Micropatterned Displays.

ACS nano·2026
See all related articles

Chemically induced self-assembly of fusion proteins created enzyme nanorings. Nanoring size, controlled by peptide linkers, influenced catalytic efficiency, offering a new way to tune enzyme activity.

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Enzyme Engineering

Background:

  • Enzyme activity is crucial in biological processes.
  • Controlling enzyme structure and function at the nanoscale is an emerging field.
  • Dihydrofolate reductase (DHFR) and Histidine triad nucleotide binding 1 (Hint1) are key enzymes.

Purpose of the Study:

  • To develop a method for creating enzyme nanostructures.
  • To investigate the relationship between nanostructure dimensions and enzyme catalytic efficiency.
  • To explore the potential of supermolecular enzyme assemblies for controlling biochemical reactions.

Main Methods:

  • Chemically induced self-assembly of dihydrofolate reductase (DHFR)-histidine triad nucleotide binding 1 (Hint1) fusion proteins.
  • Static light scattering and atomic force microscopy to characterize nanoring dimensions.

More Related Videos

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Related Experiment Videos

Last Updated: Jun 25, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

  • Assessment of catalytic efficiency in relation to nanoring size.

    • Main Results:

    • Successfully prepared nanostructures, specifically nanorings, using DHFR-Hint1 fusion proteins.
    • Nanoring size (10-70 nm diameter, 64-740 kDa) was tunable by altering the peptide linker length and composition.
    • Catalytic efficiency of the nanorings demonstrated a clear dependence on their size.

    Conclusions:

    • Enzyme nanorings can be reliably prepared via self-assembly of engineered fusion proteins.
    • The size of these enzyme nanostructures directly impacts their catalytic performance.
    • Supermolecular assembly offers a novel strategy for precise control over enzyme function.