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A novel cis-acting element that controls transcription of human immunodeficiency virus type 1 DNA, depending on cell
Y Nakanishi1, Y Masamune, N Kobayashi
1Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Abstract:
cis-acting elements for the transcription of human immunodeficiency virus type 1 DNA were analyzed in cell-free transcription and DNA transfection assays. Besides previously identified cis elements, a region adjacent to the enhancer element was found to regulate transcription in both assays. Loss of this region caused 4.3- and 1.6-fold transcription inhibition in a transfection assay with a T-cell line, MOLT-4, and a monocyte line, U937, respectively, whereas the same region appeared to function negatively with other T-cell lines, MT-4 and Jurkat. These results suggest that this novel cis element regulates the transcription of proviral DNA in a cell-type-specific manner.