Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pathway selection between click and acyl transfer reactions driven by aminoacyl phosphates.

Nature communications·2026

Same author

Tailored Phosphate Leaving Groups Direct Pathway-Dependent Self-Assembly.

Journal of the American Chemical Society·2026

Same author

Core-shell 3D printed biodegradable calcium phosphate cement-Alginate scaffolds for possible bone regeneration applications.

Frontiers in drug delivery·2025

Same author

Corrigendum: Core-shell 3D printed biodegradable calcium phosphate cement - Alginate scaffolds for possible bone regeneration applications.

Frontiers in drug delivery·2025

Same author

Chirality Makes or Breaks Chemically Driven Self-Assembly.

Angewandte Chemie (International ed. in English)·2025

Same author

Self-Assembling Amphiphilic ABA Triblock Copolymers of Hyperbranched Polyglycerol with Poly(tetrahydrofuran) and Their Nanomicelles as Highly Efficient Solubilization and Delivery Systems of Curcumin.

International journal of molecular sciences·2025

Same journal

Vertically Stacked Indium Gallium Zinc Oxide-Based Three-Dimensional Integrated Circuits.

ACS nano·2026

Same journal

Tunable Nanoparticle Thin-Film Reveals Distance Dependence of Auger-Mediated Radiation Enhancement in Diffuse Midline Glioma.

ACS nano·2026

Same journal

G-Quadruplex Network Engineering in Ionogels: Realizing Robust Biosensing Interfaces for Plant Electrophysiology.

ACS nano·2026

Same journal

Announcing the 2026 <i>ACS Nano</i> Lectureship and <i>ACS Nano</i> Impact Award Laureates.

ACS nano·2026

Same journal

Ultrafast Self-Assembly of Zeolitic Imidazolate Framework-8 Enables Antibody Orientation for Ultrasensitive Lateral Flow Immunoassays.

ACS nano·2026

Same journal

Interfacial Salt Engineering with Alkali and Ammonium Additives for Stable Pure-Blue Perovskite Light-Emitting Diodes and Micropatterned Displays.

ACS nano·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

A four-color colloidal multiplexing nanoparticle system.

Oliver Ehlert¹, Ralf Thomann, Masih Darbandi

¹Freiburg Materials Research Center, University of Freiburg, Germany.

|February 12, 2009

Summary

This summary is machine-generated.

Novel upconverting nanoparticles overcome autofluorescence issues in multicolor biological imaging. These rare earth doped nanocrystals emit four distinct colors from a single near-infrared excitation source, enabling multiplexed detection.

More Related Videos

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Related Experiment Videos

Last Updated: Jun 25, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Area of Science:

Materials Science
Nanotechnology
Biomedical Imaging

Background:

Autofluorescence in biological tissues interferes with multicolor labeling using UV or blue light excitation.
Fluorescence resonance energy transfer (FRET) can also affect multicolor imaging results.
Need for advanced imaging probes that circumvent autofluorescence and FRET limitations.

Purpose of the Study:

To develop novel near-infrared (IR) to visible (vis) upconverting nanoparticles (UCNPs).
To demonstrate multicolor emission from UCNPs using a single excitation source.
To enable robust multiplexed imaging in biological samples.

Main Methods:

Synthesis of rare earth metal-doped upconverting nanoparticles.
Silica encapsulation for phase transfer into polar solvents.
Characterization using transmission electron microscopy (TEM), X-ray diffraction (XRD), and photoluminescence (PL) spectroscopy.

Main Results:

Successfully synthesized UCNPs with tunable multicolor emission.
Achieved spectral separation of four distinct colors from a single 980 nm excitation source.
Demonstrated feasibility for multiplexed imaging applications.

Conclusions:

The developed IR-to-vis UCNPs offer a promising alternative to conventional fluorescent probes.
These UCNPs effectively mitigate autofluorescence and FRET interference in biological imaging.
The technology enables sensitive and specific multicolor imaging for advanced biomedical research.