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Related Experiment Video

Updated: Jun 25, 2026

Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Photobleaching absorbed Rhodamine B to improve temperature measurements in PDMS microchannels.

Tomasz Glawdel1, Zeyad Almutairi, Shuwen Wang

  • 1Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Canada.

Lab on a Chip
|February 12, 2009
PubMed
Summary

Photobleaching removes Rhodamine B dye absorption in microfluidic channels, improving temperature measurement accuracy. This technique enhances fluorescence thermometry by eliminating signal interference from channel walls.

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Area of Science:

  • Microfluidics
  • Optical Measurement Techniques
  • Materials Science

Background:

  • Rhodamine B fluorescence thermometry is vital for microfluidic temperature sensing.
  • Absorption of Rhodamine B into polydimethylsiloxane (PDMS) channel walls leads to signal interference and inaccurate temperature readings.
  • Existing methods lack effective solutions for Rhodamine B absorption in microchannels.

Purpose of the Study:

  • To develop and demonstrate a simple, effective method to eliminate Rhodamine B signal absorption in PDMS microchannels.
  • To improve the accuracy of fluorescence thermometry in microfluidic devices.
  • To validate the photobleaching technique for temperature field analysis in microfluidic systems.

Main Methods:

  • Utilized a high-intensity light source for photobleaching to remove absorbed Rhodamine B.

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  • Applied the photobleaching technique prior to image acquisition for thermometry.
  • Fabricated a Y-channel microfluidic chip using PDMS/glass for temperature field studies.

    • Main Results:

    • Successfully removed the interfering fluorescent signal from absorbed Rhodamine B.
    • Demonstrated accurate temperature measurements after applying the photobleaching method.
    • Visualized the temperature field at the convergence of hot and cold streams in the microchannel.

    Conclusions:

    • Photobleaching is an effective strategy to mitigate Rhodamine B absorption issues in PDMS microfluidics.
    • This technique significantly enhances the reliability of Rhodamine B fluorescence thermometry.
    • The method provides a practical solution for precise temperature mapping in microfluidic devices.