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Updated: Jun 25, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
Published on: August 9, 2011
Optimized reconstruction algorithm for helical CT with fractional pitch between 1PI and 3PI
Alexander Katsevich1, Alexander A Zamyatin, Michael D Silver
1Department of Mathematics, University of Central Florida, Orlando, FL 32816 USA. akatsevi@mail.ucf.edu
Abstract:
We propose an approximate approach to use redundant data outside the 1PI window within the exact Katsevich reconstruction framework. The proposed algorithm allows a flexible selection of the helical pitch, which is useful for clinical applications. Our idea is an extension of the one proposed by KOhler, Bontus, and Koken (2006). It is based on optimizing the contribution weights of convolution families used in exact Katsevich 3PI algorithms, so that the total weight of each Radon plane is as close to 1 as possible. Optimization is based on solving a least squares problem subject to linear constrains. Numerical evaluation shows good noise and artifact reduction properties of the proposed algorithm.

