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Updated: Jun 25, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
Functions and regulatory mechanisms of Gq-signaling pathways
Norikazu Mizuno1, Hiroshi Itoh
1Department of Cell Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan.
Abstract:
Gq family members of heterotrimeric G protein activate beta isoforms of phospholipase C that hydrolyzes phosphatidylinositol phosphate to diacylglycerol and inositol trisphosphate, leading to the protein kinase C activation and intracellular Ca(2+) mobilization, respectively. To understand the functions and regulatory mechanisms of Gq-signaling pathways, we first introduce the Galphaq-interacting proteins, which function as the effectors and the modulators of Gq. Next, we describe the Pasteurella multocida toxin and YM-254890, which are useful tools to investigate Gq signaling as activator and inhibitor, respectively. Finally, we discuss the physiological function of Gq in developmental brain, especially in neural progenitor cells.
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