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iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Related Experiment Video

Updated: Jun 25, 2026

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Published on: June 28, 2013

Functional cardiomyocytes derived from human induced pluripotent stem cells.

Jianhua Zhang¹, Gisela F Wilson, Andrew G Soerens

¹Department of Medicine, University of Wisconsin, WiCell Research Institute, Madison, WI 53792-3248, USA.

Circulation Research

|February 14, 2009

Summary

Human induced pluripotent stem (iPS) cells can differentiate into functional cardiomyocytes, similar to human embryonic stem (ES) cells. This finding supports iPS cells as a viable source for cardiac repair and cardiovascular research.

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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

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Last Updated: Jun 25, 2026

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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

Area of Science:

Stem cell biology
Cardiovascular research
Regenerative medicine

Background:

Human induced pluripotent stem (iPS) cells offer potential for cardiovascular applications.
Demonstrating functional cardiomyocyte differentiation from human iPS cells is crucial.

Purpose of the Study:

To compare the cardiac differentiation potential of human iPS cells with human embryonic stem (ES) cells.
To characterize the functional and molecular properties of iPS cell-derived cardiomyocytes.

Main Methods:

Embryoid body (EB) method for differentiation of iPS and ES cells.
RT-PCR for cardiac gene expression analysis.
5-bromodeoxyuridine labeling for proliferation assessment.
Immunocytochemistry for sarcomeric organization.
Electrophysiology studies for action potential characteristics.

Main Results:

Comparable time course of contracting EBs between iPS and ES cells.
Similar cardiac gene expression patterns and sarcomeric organization in derived cardiomyocytes.
iPS and ES cell-derived cardiomyocytes showed similar proliferation and electrophysiological properties, including responsiveness to beta-adrenergic stimulation.
Blunted downregulation of pluripotency genes in iPS cells due to residual transgene expression.

Conclusions:

Human iPS cells can differentiate into functional cardiomyocytes.
iPS cells are a viable autologous cell source for cardiac repair.
iPS cells represent a powerful tool for cardiovascular research.