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Related Experiment Video

Updated: Jun 25, 2026

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Published on: September 23, 2015

Central 5,7-dihydroxytryptamine lesions decrease hippocampal glucocorticoid and mineralocorticoid receptor messenger

J R Seckl¹, K L Dickson, G Fink

¹University of Edinburgh, Department of Medicine, Western General Hospital, Edinburgh EH4 2XU, UK.

Journal of Neuroendocrinology

|February 14, 2009

Summary

This summary is machine-generated.

Serotonin (5-HT) neurons regulate corticosteroid receptor expression in the rat hippocampus. Lesioning these neurons significantly decreased glucocorticoid and mineralocorticoid receptor mRNA levels in specific hippocampal subregions.

Related Experiment Videos

Last Updated: Jun 25, 2026

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Published on: September 23, 2015

Area of Science:

Neuroscience
Endocrinology
Molecular Biology

Background:

Corticosteroids impact the hippocampus via glucocorticoid and mineralocorticoid receptors.
The hippocampus has extensive serotoninergic (5-HT) innervation that interacts with corticosteroid-sensitive cells.
The precise roles of each receptor type in corticosteroid action are not fully understood.

Purpose of the Study:

To investigate the effect of 5-HT neuron lesions on glucocorticoid and mineralocorticoid receptor mRNA expression in the rat hippocampus.
To determine how serotoninergic innervation influences corticosteroid receptor regulation within the hippocampus.

Main Methods:

Used in situ hybridization histochemistry to measure receptor mRNA expression.
Administered intracerebroventricular 5,7-dihydroxytryptamine to lesion 5-HT neurons in rats.
Quantified glucocorticoid and mineralocorticoid receptor mRNA levels in different hippocampal subregions.

Main Results:

Glucocorticoid receptor mRNA was highly expressed in dentate gyrus, CA1, and CA2, with lower levels in CA3 and CA4.
5-HT neuron lesions significantly decreased glucocorticoid receptor mRNA in the dentate gyrus (76%), CA1 (42%), and CA2 (52%).
Mineralocorticoid receptor mRNA levels were similar across subregions in controls. Lesions significantly decreased mineralocorticoid receptor mRNA in CA3 (56%) and CA4 (45%).

Conclusions:

Serotoninergic innervation plays a significant role in regulating hippocampal corticosteroid receptor mRNA expression.
Specific hippocampal subregions show differential regulation of glucocorticoid and mineralocorticoid receptors by 5-HT neurons.
These findings highlight the intricate interplay between the serotoninergic system and the corticosteroid system in the hippocampus.