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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Performance evaluation of a high-field Orbitrap mass analyzer
Alexander Makarov1, Eduard Denisov, Oliver Lange
1Thermo Fisher Scientific, Bremen, Germany. alexander.makarov@thermofisher.com
Journal of the American Society for Mass Spectrometry
|February 14, 2009
Summary
A novel Orbitrap mass analyzer design enhances resolving power by reducing electrode gaps, achieving over 600,000 resolution. This advancement improves protein analysis and dynamic range for mass spectrometry applications.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Instrumentation
Background:
- The Orbitrap mass analyzer is a key technology in high-resolution mass spectrometry.
- Limitations in resolving power and dynamic range can impact complex sample analysis.
- Optimizing instrument design is crucial for advancing analytical capabilities.
Purpose of the Study:
- To introduce a new Orbitrap mass analyzer design.
- To investigate the impact of reduced electrode gaps on ion oscillation frequencies and resolving power.
- To evaluate the performance of the new design in terms of resolution, isotopic mass accuracy, and dynamic range.
Main Methods:
- A modified Orbitrap analyzer with decreased electrode gap was constructed.
- Experimental measurements of resolving power, isotopic resolution, and dynamic range were performed.
- Comparison of performance metrics with a standard Orbitrap analyzer.
Main Results:
- Achieved a full width at half maximum (FWHM) resolving power exceeding 350,000 at m/z 524 and 600,000 at m/z 195.
- Demonstrated isotopic resolution for proteins above 40 kDa.
- Obtained a single-shot dynamic range of 25,000 with minimal mass shifts due to space charge.
Conclusions:
- The new Orbitrap design significantly enhances resolving power and dynamic range.
- Reduced electrode gaps lead to higher ion oscillation frequencies and improved performance.
- This advancement offers greater capabilities for high-resolution mass spectrometry applications.
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