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Laboratory Scale Production and Purification of a Therapeutic Antibody
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Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression

Masaki Magari1, Takahiro Aya, Mika Ikeda

  • 1Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.

Journal of Bioscience and Bioengineering
|February 17, 2009
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...