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Updated: Jun 25, 2026
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
Published on: July 31, 2017
[Expression of Aurora-B in human glioma tissue and its significance]
Li-bo Sun1, Shuang-lin Fu, Yi-nan Luo
1Department of Neurosurgery, First Affiliated Hospital of Jilin University, Changchun 130021, China.
Objective:
To study the expression of Aurora-B in human glioma tissue and its significance.
Methods:
The total RNA was extracted from 41 human glioma tissues and 11 normal brain tissues by Trizol reagent. After reverse transcription of the total RNA into cDNAs, Aurora-B mRNA expressions in these samples were detected by quantitative real-time PCR. The protein expression in these samples was detected using immunohistochemical staining.
Results:
Aurora-B mRNA and protein expressions were significantly increased in glioma tissues as compared with those in normal brain tissues.
Conclusion:
Aurora-B mRNA and protein show markedly higher expressions in glioma tissue, suggesting that Aurora-B may be one of the malignant biomarkers in the pathogenesis and progression of human glioma.
