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[Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis]
Xiao-xia Wang1, Xiao-bao Yao, Xian-sheng Ji
1Department of Otolaryngology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China. xiaoxiawang9139@sina.com
Objective:
To investigate the apoptosis-inducing effect of caspase-3 on human nasopharyngeal carcinoma cells (CNE2).
Methods:
Recombinant caspase-3 was subcloned into the eukaryotic expression vector PEGFP-C1 containing the reporter gene using DNA recombinant technique. CNE2 cells were transfected with the recombinant caspase-3 gene via lipofectamine 2000 and the expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction. The cell morphological changes were observed under fluorescence microscope and electron microscope and the cell survival rate after the transfection was assessed by MTT assay.
Results:
Transfection with the recombinant caspase-3 gene induced significant apoptosis in CNE2 cells, which exhibited obvious morphological changes typical of apoptotic cells.
Conclusion:
The recombinant caspase-3 gene can inhibit the growth and effectively induce apoptosis of CNE2 cells.
