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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 25, 2026

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Diffraction Phase Cytometry: blood on a CD-ROM.

Mustafa Mir¹, Zhuo Wang, Krishnarao Tangella

¹Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

|February 17, 2009

Summary

This summary is machine-generated.

Diffraction Phase Cytometry (DPC) offers a new way to analyze red blood cells in whole blood. This high-throughput imaging technique provides detailed cell measurements for potential low-cost blood screening devices.

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Published on: January 7, 2019

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Last Updated: Jun 25, 2026

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Isolation of CD4⁺ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

Area of Science:

Biomedical Engineering
Optical Imaging
Hematology

Background:

Existing cytometers lack comprehensive geometrical parameter retrieval for erythrocytes.
Quantitative phase imaging offers potential for advanced blood analysis.

Purpose of the Study:

To demonstrate Diffraction Phase Cytometry (DPC) as a high-throughput quantitative phase imaging modality for whole blood smear analysis.
To develop a compact instrument for quantitative, physiologically relevant measurements of erythrocytes.

Main Methods:

Utilized a commercial CD as a sample substrate.
Employed dynamic spatial filtering with a liquid crystal spatial light modulator.
Developed a compact, single-shot, full-field imaging instrument.

Main Results:

Imaged 1,537 live human erythrocytes in whole blood without prior preparation.
Retrieved comprehensive geometrical parameters including cell volume and surface area.
Determined the minimum cylindrical diameter for erythrocyte passage, crucial for oxygen delivery.

Conclusions:

Diffraction Phase Cytometry (DPC) is a sensitive and effective method for analyzing erythrocytes.
The developed system proves the concept for an inexpensive lab-on-a-chip blood screening device.
DPC provides unique insights into erythrocyte morphology beyond conventional cytometers.