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Updated: Jun 25, 2026

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration
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Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration

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Estrogen replacement avoids the decrease of bladder innervations in ovariectomized adult virgin rats: in vivo

Rogerio de Fraga1, Paulo Palma, Miriam Dambros

  • 1Universidade Federal do Paraná, Rua Bruno Filgueira 2211, AP 1301 CEP 80730-380 Curitiba, PR, Brazil. ogeriodefraga@uol.com.br

International Urogynecology Journal and Pelvic Floor Dysfunction
|February 18, 2009
PubMed
Summary

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Estrogen deprivation significantly reduces bladder nerve fibers by 65%. Estradiol replacement therapy may help restore bladder innervation in ovariectomized rats, impacting bladder function.

Area of Science:

  • Urology
  • Neuroscience
  • Endocrinology

Background:

  • Estrogen plays a crucial role in maintaining physiological functions.
  • Ovariectomy in rats leads to estrogen deficiency, mimicking menopause.
  • Bladder innervation is vital for normal bladder function.

Purpose of the Study:

  • To investigate the impact of long-term estrogen deprivation on bladder nerve fibers.
  • To evaluate the effect of estradiol replacement on bladder innervation in ovariectomized rats.

Main Methods:

  • Wistar rats underwent bilateral ovariectomy or sham surgery.
  • Groups received either sesame oil or 17β-estradiol replacement for 90 days.
  • Nerve fibers in bladder tissue were quantified using S-100 staining and the G-50 grid system.

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Published on: June 7, 2012

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Main Results:

  • Ovariectomized rats without estradiol replacement showed a 65% decrease in bladder nerve fibers (p < 0.001).
  • Estradiol replacement in ovariectomized rats demonstrated potential in mitigating nerve fiber loss.

Conclusions:

  • Long-term estrogen deprivation significantly alters bladder innervation.
  • Estrogen deficiency negatively impacts the density of nerve fibers in the bladder wall.