Hagfish natriuretic peptide changes urine flow rates and vascular tensions in a hagfish

Since the first report of their natriuretic effect on mammalian kidneys the relative influences of natriuretic peptides (NPs) on volume and salt regulation in vertebrates have been debated. As marine osmoconformers, with plasma ionic concentrations similar to seawater, the actions of NPs on hagfishes may provide information on their primordial role. A synthetic natriuretic peptide derived from Eptatretus burgeri (hNP) increased urine production rates in E. cirrhatus at 3x10(-8) M. It also contracted afferent branchial and segmental arteries at low concentrations (1x10(-10) M) and relaxed them at 3x10(-8) M. Thus, hNP has a renal effect and at higher concentrations causes vascular relaxation suggesting a role in volume regulation and the prevention of cardiac overloading. Rat ANP (rANP) stimulated sodium efflux from both isolated, perfused gill pouches and the whole animal. rANP also reduced subcutaneous sinus haematocrit relative to that in the ventral aorta, which is consistent with a vasodilatory role.