Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

A Gradient-generating Microfluidic Device for Cell Biology
11:05

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

Generalized serial dilution module for monotonic and arbitrary microfluidic gradient generators.

Kangsun Lee1, Choong Kim, Byungwook Ahn

  • 1SMALL (Nanobio Sensors and MicroActuators Learning Lab), Department of Electrical Engineering, University at Buffalo, the State University of New York (SUNY at Buffalo), Buffalo, NY 14260, USA.

Lab on a Chip
|February 19, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct Pattern-to-Curve PDMS-Based Microstructures Fabrication via Thermal Air Expansion for Micro-Optics.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Evaporation-driven digital ELISA with micro-droplet arrays for ultrafast detection of low-abundance proteins.

Biosensors & bioelectronics·2025
Same author

Meso-macroporous hydrogel for direct litre-scale isolation of extracellular vesicles.

Nature nanotechnology·2025
Same author

Microencapsulation system for scalable differentiation of peripheral motor neurons from human induced pluripotent stem cells.

Biomaterials science·2025
Same author

Corrigendum to "Implementation of an ultra-sensitive microwell-based electrochemical sensor for the detection of Alzheimer's disease" [Biosens. Bioelectron. (2024) 247 115898].

Biosensors & bioelectronics·2025
Same author

Achieving Quasi Intrinsic MoS<sub>2</sub> Performance via Liquid-Based Neutralization of Material and Interface Defects with H<sup>+</sup> Protons.

ACS applied materials & interfaces·2024
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

We developed a universal microfluidic dilution module to precisely control chemical concentrations. This technology enables the generation of various gradient shapes for diverse biological and chemical applications.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Chemical Engineering

Background:

  • Microfluidic devices are crucial for precise control of chemical and biological processes.
  • Generating complex concentration gradients in microfluidic systems remains a challenge for many applications.

Purpose of the Study:

  • To propose a generalized serial dilution module for universal microfluidic concentration gradient generators.
  • To develop a mathematical model for predicting and controlling concentration and flow rate gradients.

Main Methods:

  • A generalized serial dilution module with N cascaded-mixing stages was designed.
  • A mathematical model based on electrical circuit analogies (pressure drop-voltage, flow rate-current, flow resistance-electrical resistance) was developed.
  • Channel lengths were adjusted to control fluidic resistance and thus flow rates.

More Related Videos

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
11:23

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Published on: February 23, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

A Gradient-generating Microfluidic Device for Cell Biology
11:05

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
11:23

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Published on: February 23, 2017

Main Results:

  • A generalized mathematical model was established, with channel length as the sole variable parameter.
  • Three types of microfluidic gradient generators (linear, logarithmic, Gaussian) were successfully designed and tested using N=4 stages.
  • The module demonstrated capability in generating universal monotonic and arbitrary non-linear concentration gradients and flow rate gradients.

Conclusions:

  • The proposed generalized serial dilution module offers a universal technology for generating complex concentration gradients in microfluidic devices.
  • This technology simplifies gradient generation, enabling widespread use in chemical and biological research for studying gradient-dependent phenomena.
  • The approach facilitates the creation of both concentration and flow rate gradients in a stepwise manner.