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Updated: Jun 25, 2026
A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat
Published on: December 28, 2013
Role of NMDA receptor upon [14C]acetate uptake into intact rat brain
Shinichiro Hirose1, Sotaro Momosaki, Rie Hosoi
1Division of Health Sciences, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. hirose@sahs.med.osaka-u.ac.jp
Objective:
To clarify the role of N-methyl-D: -aspartate (NMDA) receptors upon [(14)C]acetate uptake in the rodent central nervous system (CNS), ibotenic acid (IBO) was infused into the right striatum of the rat brain.
Methods:
Autoradiograms of [(14)C]acetate uptake in the brain for 2 h following the infusion of IBO (10 microg/microl) were obtained in both non-treated and MK-801 (1 mg/kg, i.v.) pretreated rats. The effect of MK-801 on [(14)C]acetate uptake in the normal rat brain was also studied.
Results:
Infusion of IBO significantly decreased [(14)C]acetate uptake in the infused side of the striatum. The expression of monocarboxylate transporter-1 was not altered, suggesting that the activity of tricarboxylic acid (TCA) cycle in glial cells might be depressed. Pretreatment with MK-801 completely blocked the decreasing effect of IBO on [(14)C]acetate uptake. MK-801 also increased [(14)C]acetate uptake in the whole brain of normal rats.
Conclusions:
These results indicate the important roles of NMDA receptors on [(14)C]acetate uptake in the intact rat brain.
