Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heat treatment combined with hybrid exercises retraining mitigates cellular markers of protein turnover after hindlimb suspension in male mice: A pilot study.

Experimental physiology·2026
Same author

Baroreceptor Mechanotransduction: Diverse Sensors, Unified Signals.

Hypertension (Dallas, Tex. : 1979)·2026
Same author

Soluble CD146 reflects altered endothelial and metabolic homeostasis in peripheral artery disease.

Angiogenesis·2026
Same author

CD146 extracellular vesicles accumulate at atherosclerotic lesions, reducing inflammation and atheroma burden.

Atherosclerosis·2026
Same author

Extracellular vesicles in atherosclerotic cardiovascular disease: mechanisms and therapeutic implications.

European heart journal·2026
Same author

Fast mechanosensitive and Ca<sup>2+</sup>-dependent reorientation of motile cilia basal bodies in the placozoan Trichoplax.

Current biology : CB·2026

Related Experiment Video

Updated: Jun 25, 2026

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

CD146 and its soluble form regulate monocyte transendothelial migration.

Nathalie Bardin1, Marcel Blot-Chabaud, Nicolas Despoix

  • 1UMR-S 608 INSERM, Laboratoire d'Hématologie et d'Immunologie, Université de la Méditerranée, UFR de Pharmacie, 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France. nathalie.bardin@pharmacie.univ-mrs.fr

Arteriosclerosis, Thrombosis, and Vascular Biology
|February 21, 2009
PubMed
Summary

Tumor necrosis factor (TNF) regulates CD146, a molecule crucial for monocyte transmigration during inflammation. Both CD146 and its soluble form (sCD146) facilitate this process, with CD146 potentially binding to an unknown monocyte ligand.

More Related Videos

Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization
07:45

Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization

Published on: January 23, 2019

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization
07:45

Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization

Published on: January 23, 2019

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Cell adhesion molecules are critical for leukocyte transmigration during inflammation.
  • CD146, located at interendothelial junctions, influences cell cohesion and permeability.
  • The specific role of CD146 in monocyte transmigration remained unclear.

Purpose of the Study:

  • To investigate the role of CD146 in monocyte transendothelial migration.
  • To determine how inflammatory cytokines, specifically TNF, affect CD146 expression and function.
  • To elucidate the mechanism by which CD146 facilitates monocyte migration.

Main Methods:

  • Human umbilical vein endothelial cells (HUVECs) were treated with TNF to assess CD146 expression and release of soluble CD146 (sCD146).
  • Metalloproteinase activity was evaluated for its role in sCD146 release.
  • Monocyte transmigration assays were performed using THP1 cells and primary monocytes, with inhibition by anti-CD146 antibodies and CD146 siRNA.
  • Binding assays were conducted to analyze interactions between sCD146, monocytes, and HUVECs.

Main Results:

  • TNF upregulated CD146 expression at the HUVEC junction and apical membrane via synthesis and redistribution.
  • TNF increased sCD146 release through a metalloproteinase-dependent pathway.
  • Inhibition of CD146 in HUVECs reduced monocyte transmigration.
  • sCD146 bound to both monocytes and HUVECs, enhancing monocyte transmigration in a dose-dependent manner.
  • sCD146 did not exhibit homophilic binding, suggesting heterophilic interaction with monocytes.

Conclusions:

  • CD146 expression and localization are modulated by the inflammatory cytokine TNF.
  • Both endothelial CD146 and soluble sCD146 play significant roles in monocyte transendothelial migration.
  • Endothelial CD146 likely interacts heterophilically with an unidentified ligand on monocytes to facilitate transmigration.