Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Elevated Plus Maze for Mice

Elevated Plus Maze for Mice

Published on: December 22, 2008

Elevated plus maze for mice.

Munekazu Komada¹, Keizo Takao, Tsuyoshi Miyakawa

¹Genetic Engineering and Functional Genomics Unit, Kyoto University Graduate School of Medicine. komada@behav.hmro.med.kyoto-u.ac.jp

Journal of Visualized Experiments : Jove

|February 21, 2009

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative analysis of procedural variations in the social defeat stress paradigm in mice: effects of post-defeat housing conditions and aggressor exposure duration.

Molecular brain·2026

Same author

Sleep Deprivation and Neuronal Hyperexcitation Share Transcriptomic Signatures.

Neuropsychopharmacology reports·2026

Same author

Clustered protocadherinβs contribute to configuring functional cell populations in hippocampal-cortical regions.

Neuroscience research·2026

Same author

CD5L promotes phagocytic removal of amyloid β oligomers and improves cognitive function in a mouse model of Alzheimer's disease.

Cell reports·2026

Same author

Iba1 deficiency impairs microglial synaptic remodeling and neuronal survival after axonal injury.

Journal of neuroinflammation·2026

Same author

Alternative microexon splicing code for a four-amino acid peptide of PTPRD governs behavioral development.

Proceedings of the National Academy of Sciences of the United States of America·2026

Standardizing the elevated plus maze test protocol is crucial for understanding mouse behavior genetics. This study provides a detailed video protocol to improve reproducibility and data comparison across laboratories for mutant mouse anxiety research.

Area of Science:

Neuroscience
Behavioral Genetics
Animal Behavior

Background:

The mouse genome is sequenced, but most brain gene functions remain unknown.
Mutant mouse behavioral analysis is key to understanding gene function in behavior.
The elevated plus maze test is a standard method for assessing anxiety-like behavior in mice.

Purpose of the Study:

To present a detailed video protocol for the elevated plus maze test.
To promote standardization of the elevated plus maze test across laboratories.
To facilitate comparison of behavioral phenotypes in mutant mice.

Main Methods:

Detailed video demonstration of the elevated plus maze test protocol.
Assessment of over 90 strains of mutant mice using the standardized protocol.

More Related Videos

The Successive Alleys Test of Anxiety in Mice and Rats

The Successive Alleys Test of Anxiety in Mice and Rats

Published on: June 17, 2013

Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements

Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Elevated Plus Maze for Mice

Elevated Plus Maze for Mice

Published on: December 22, 2008

The Successive Alleys Test of Anxiety in Mice and Rats

The Successive Alleys Test of Anxiety in Mice and Rats

Published on: June 17, 2013

Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements

Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements

Published on: January 7, 2019

Development of a public database for disclosed data.

Main Results:

A comprehensive video protocol for the elevated plus maze test is provided.
The protocol has been successfully applied to over 90 mutant mouse strains.
Standardization will enable better comparison of anxiety-like behaviors.

Conclusions:

Standardized protocols are essential for reliable behavioral genetics research.
The provided video enhances understanding and reproducibility of the elevated plus maze test.
This initiative will improve the study of genetic mechanisms underlying behavior.