Related Experiment Video
Updated: Jun 25, 2026
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Published on: February 22, 2020
Alginate molecular mass produced by Azotobacter vinelandii in response to changes of the O2 transfer rate in
A Díaz-Barrera1, P Silva, R Avalos
1Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Casilla, 4059 Valparaiso, Chile. alvaro.diaz@ucv.cl
Abstract:
Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g l(-1)) was observed. At a constant growth rate (0.1 h(-1)), the mean molecular mass of the alginate was strongly influenced by changes in the OTR, varying from 860 to 1,690 kDa. These results support a possible relationship between alginate polymerization-depolymerization process and the O(2) uptake rate.
Related Concept Videos
02:35DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
01:18Bioreactor Controls-II