Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Insertion of Multi-pass Transmembrane Proteins in the RER01:29

Insertion of Multi-pass Transmembrane Proteins in the RER

The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Electrophilic Allergen Screening Assay for Detection of Key Event 1 of the Skin Sensitization Adverse Outcome Pathway.

Toxics·2026
Same author

Linker histones consolidate heterogenous nucleosome fiber contacts by linking together multiple nucleosomes.

Nature communications·2026
Same author

Comparative Toxicity of Seven Aqueous Film-Forming Foam to In Vitro Systems and Mus.

Environmental toxicology and chemistry·2023
Same author

Integrating "One Health" Concepts in the Design of Sustainable Systems for Environmental Use.

Toxics·2023
Same author

The solid and liquid states of chromatin.

Epigenetics & chromatin·2021
Same author

Condensed Chromatin Behaves like a Solid on the Mesoscale In Vitro and in Living Cells.

Cell·2020

Related Experiment Video

Updated: Jun 25, 2026

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

Recent advances in MeCP2 structure and function.

Kristopher C Hite1, Valerie H Adams, Jeffrey C Hansen

  • 1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA. krishite@rams.colostate.edu

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|February 24, 2009
PubMed
Summary
This summary is machine-generated.

Mutations in methyl DNA binding protein 2 (MeCP2) cause Rett syndrome. New data reveal MeCP2 is a multifunctional regulator, not just a silencer, impacting chromatin and transcription.

More Related Videos

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
08:47

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
08:47

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Area of Science:

  • Molecular Biology
  • Neurogenetics
  • Biochemistry

Background:

  • Mutations in methyl DNA binding protein 2 (MeCP2) are linked to Rett syndrome (RTT).
  • The precise cellular functions of MeCP2 remain incompletely understood.
  • MeCP2 was traditionally viewed as a gene silencer with distinct functional domains.

Purpose of the Study:

  • To review recent advancements in understanding MeCP2 structure and function.
  • To analyze new evidence suggesting a broader regulatory role for MeCP2.
  • To explore the implications of MeCP2's multifunctionality in RTT.

Main Methods:

  • Review of recent structural biochemistry studies on MeCP2.
  • Analysis of cell-based experiments investigating MeCP2's transcriptional role.
  • Correlation of clinical data with MeCP2 domain-specific mutations.

Main Results:

  • MeCP2 possesses a more complex structure-function relationship than previously thought.
  • Evidence suggests MeCP2 actively regulates transcription, not solely silences genes.
  • MeCP2 functions as a multifunctional nuclear protein involved in chromatin architecture and RNA splicing.

Conclusions:

  • MeCP2 is a key regulator of various nuclear processes, including active transcription.
  • All structural domains of MeCP2 are essential for its proper cellular function.
  • Understanding MeCP2's multifaceted roles is crucial for RTT pathology.