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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

Analytical strategies for phosphoproteomics.

Tine E Thingholm1, Ole N Jensen, Martin R Larsen

  • 1KMEB, Department of Endocrinology, Odense University Hospital, Odense M, Denmark. tthingholm@health.sdu.dk

Proteomics
|February 24, 2009
PubMed
Summary

Understanding protein phosphorylation is crucial for cell signaling and disease research. This review highlights sensitive mass spectrometry and affinity methods for analyzing low-abundance phosphoproteins.

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Last Updated: Jun 25, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

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Published on: October 12, 2009

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Protein phosphorylation regulates critical cellular processes like proliferation, differentiation, and apoptosis.
  • Dysregulated signaling pathways due to altered phosphorylation are linked to various diseases.
  • Phosphorylation is a transient modification, and phosphoproteins are typically low in abundance, complicating analysis.

Purpose of the Study:

  • To provide an overview of analytical strategies for phosphoprotein characterization.
  • To emphasize affinity-based enrichment methods for phosphoproteins and phosphopeptides.
  • To discuss recent applications of these methods in cell biology.

Main Methods:

  • Mass spectrometry (MS) is the primary technique for phosphoproteome analysis.
  • Phospho-specific enrichment methods are crucial for detecting low-abundance phosphoproteins.
  • Affinity-based methods are highlighted for phosphoprotein and phosphopeptide enrichment prior to MS.

Main Results:

  • Sensitive and specific strategies are required for comprehensive phosphoproteome analysis.
  • Affinity enrichment methods significantly enhance the detection of phosphoproteins.
  • These methods have broad applications in understanding cell biological processes.

Conclusions:

  • Effective phosphoproteomic analysis relies on sensitive enrichment techniques coupled with mass spectrometry.
  • Understanding protein phosphorylation is vital for deciphering cellular functions and disease mechanisms.
  • Advancements in affinity enrichment methods are expanding the scope of phosphoproteomics in cell biology.