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Related Experiment Video

Updated: Jun 25, 2026

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

Activators of PPARs and LXR decrease the adverse effects of exogenous glucocorticoids on the epidermis.

Marianne Demerjian¹, Eung-Ho Choi, Mao-Qiang Man

¹Department of Dermatology, UCSF and VA Medical Center, San Francisco, CA 94121, USA.

Experimental Dermatology

|February 25, 2009

Summary

This summary is machine-generated.

Glucocorticoids (GC) harm skin by reducing cell growth and differentiation. PPAR and LXR activators applied with GC treatments prevented these toxic effects, protecting epidermal health.

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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

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Published on: November 15, 2013

Area of Science:

Dermatology
Molecular Biology
Pharmacology

Background:

Glucocorticoids (GC) have anti-inflammatory benefits but cause epidermal adverse effects.
These include reduced keratinocyte proliferation, differentiation, and barrier homeostasis.
Previous attempts using lipid mixtures failed to prevent GC-induced epidermal abnormalities.

Purpose of the Study:

To develop strategies preventing topical glucocorticoid (GC) toxicities on the epidermis.
To investigate the potential of Peroxisome proliferator-activated receptors (PPAR) and Liver X Receptor (LXR) activators in mitigating GC side effects.

Main Methods:

Clobetasol-treated mice were co-treated with various PPAR (alpha, beta/delta, gamma) and LXR activators.
Effects on epidermal differentiation markers (involucrin, filaggrin, loricrin) were assessed.
Keratinocyte proliferation (PCNA) and skin barrier function were evaluated.

Main Results:

PPAR alpha, gamma, and LXR activators prevented decreases in involucrin, filaggrin, and loricrin expression.
PPAR alpha, beta/delta, and LXR activators partially prevented reduced keratinocyte proliferation.
PPAR gamma and beta/delta activators improved skin barrier homeostasis.

Conclusions:

PPAR and LXR activators can prevent multiple adverse effects of topical glucocorticoids on the epidermis.
Targeting these pathways offers a promising strategy for managing GC-induced skin toxicity.