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Poly(N,N-dimethylacrylamide)-coated maghemite nanoparticles for stem cell labeling
Michal Babic1, Daniel Horák, Pavla Jendelová
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 16206 Prague 6, Czech Republic.
Bioconjugate Chemistry
|February 25, 2009
Summary
Poly(N,N-dimethylacrylamide)-coated maghemite nanoparticles enhance mesenchymal stem cell labeling and MRI contrast. These PDMAAm-coated nanoparticles show improved cellular uptake and higher T2 relaxivity for in vivo imaging applications.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Maghemite (gamma-Fe2O3) nanoparticles are synthesized via coprecipitation and oxidation.
- Polymer coating of nanoparticles is crucial for targeted applications and improved biocompatibility.
Purpose of the Study:
- To synthesize and characterize poly(N,N-dimethylacrylamide) (PDMAAm)-coated maghemite nanoparticles.
- To evaluate the efficiency of these nanoparticles for labeling mesenchymal stem cells (MSCs).
- To assess the performance of labeled MSCs in MRI imaging applications.
Main Methods:
- Coprecipitation and oxidation for maghemite synthesis, followed by radical polymerization for PDMAAm coating.
- Characterization using electron microscopy, spectroscopy (elemental analysis, ATR FTIR, AAS), and dynamic light scattering (DLS).
- Cell labeling with maghemite nanoparticles (uncoated and PDMAAm-coated) and Endorem, followed by Prussian Blue staining, TEM, T2-MR relaxometry, and iron content analysis.
Main Results:
- PDMAAm coating on maghemite nanoparticles was confirmed.
- PDMAAm-coated nanoparticles showed higher intracellular uptake efficiency in human MSCs compared to uncoated or Endorem-coated nanoparticles.
- PDMAAm-coated nanoparticles exhibited the highest T2 relaxivity, leading to improved contrast in MR imaging.
- In vivo MR imaging of PDMAAm-modified iron oxide-labeled MSCs in a rat brain demonstrated enhanced resolution.
Conclusions:
- PDMAAm-coated maghemite nanoparticles are effective agents for MSC labeling, offering superior cellular uptake and MRI contrast.
- These nanoparticles hold significant potential for advanced diagnostic and therapeutic applications in regenerative medicine and neuroimaging.
