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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
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Published on: September 18, 2013

Differential phosphoprotein labelling (DIPPL) using 32P and 33P.

Aviva M Tolkovsky1, Andreas Wyttenbach

  • 1Department of Biochemistry, University of Cambridge, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed Differential Phosphoprotein Labelling (DIPPL) to accurately study protein phosphorylation. This method uses distinct radioactive isotopes (32P and 33P) for precise analysis of phosphorylation changes in biological samples.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Differential in-gel electrophoresis (DIGE) aids protein expression analysis by pre-mixing samples.
  • Phosphorylation shifts protein isoelectric points, but DIGE analysis for these events is problematic.
  • Accurate detection of protein phosphorylation is crucial for understanding cellular signaling.

Purpose of the Study:

  • To introduce a novel differential phosphoprotein labelling technique, DIPPL.
  • To overcome limitations of existing DIGE methods for studying phosphorylation.
  • To enable precise quantification and analysis of phosphorylation changes.

Main Methods:

  • Developed DIPPL, a technique using 32Pi and 33Pi (orthophosphate) for differential labelling of two samples.
  • Mixed labelled samples and separated proteins on a single gel.
  • Utilized dual-exposure autoradiography with and without an acetate filter to distinguish 32P and 33P signals.

Main Results:

  • Demonstrated DIPPL's utility in analyzing MEK/ERK-dependent stathmin phosphorylation.
  • Successfully identified changes in stathmin phosphorylation induced by nerve growth factor (NGF).
  • Showcased the technique's ability to resolve and quantify phosphorylation events.

Conclusions:

  • DIPPL offers the advantages of DIGE for analyzing protein phosphorylation.
  • The technique provides a robust method for studying dynamic changes in phosphoproteomes.
  • DIPPL is valuable for investigating signaling pathways involving protein phosphorylation.