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Updated: Jun 25, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in
Steve M M Sweet1, Helen J Cooper
1University of Birmingham, School of Biosciences, Birmingham, UK.
Abstract:
Electron capture dissociation (ECD) allows fragmentation of the phosphopeptide backbone while keeping the labile phospho-amino acid intact. This feature of ECD fragmentation, coupled with the acquisition of mass spectra at high mass accuracy, makes ECD well-suited to phosphorylation mapping. The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses.
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