Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
08:43

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

Published on: November 30, 2012

Protein transduction method for cerebrovascular disorders.

Tomoyuki Ogawa1, Shigeki Ono, Tomotsugu Ichikawa

  • 1Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. tomoyuki_ogawa510202@yahoo.co.jp

Acta Medica Okayama
|February 28, 2009
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Possible involvement of genome-wide near-haploidy and <i>MEN1</i> mutation in the molecular pathogenesis of pituitary carcinoma: Analysis by whole exome sequencing.

Neuro-oncology advances·2026
Same author

Frailty and Severe White Matter Lesions Are Risk Factors for Surgical Treatment for Unruptured Cerebral Aneurysm in Older Adults.

Neurologia medico-chirurgica·2026
Same author

Molecular and clinical features of a Japanese medulloblastoma cohort: Subgroup-specific prognostic stratification using economical/accessible diagnostic methods.

Brain pathology (Zurich, Switzerland)·2026
Same author

Dual molecular therapy targeting tumor cell heterogeneity improves therapeutic efficacy in glioblastoma.

iScience·2025
Same author

Unveiling the Possibility of Subclinically Present Congenital Hydrocephalus Triggered by Thalamic Hemorrhage in Late-onset Years: A Case Report.

NMC case report journal·2025
Same author

Safety of Adenosine-assisted Clipping Surgery for Unruptured Cerebral Aneurysms: Interim Results of a Single-center, Single-arm Study.

NMC case report journal·2025

The 11-arginine (11R) protein transduction domain effectively delivers proteins into cerebral arteries. This novel method shows promise for treating cerebral vasospasm by enhancing cellular delivery.

Area of Science:

  • Cell Biology
  • Neuroscience
  • Biochemistry

Background:

  • Protein transduction domains (PTDs) facilitate cellular delivery.
  • The 11-arginine (11R) motif is a highly effective PTD.
  • Cerebral vasospasm (CV) remains a significant clinical challenge.

Purpose of the Study:

  • To evaluate the transduction efficiency of the 11R PTD in cerebral arteries.
  • To assess the potential of 11R-mediated delivery for treating CV.

Main Methods:

  • Fusion of enhanced green fluorescent protein (EGFP) with the 11R motif (11R-EGFP).
  • Administration of 11R-EGFP to rat basilar arteries (BA).
  • Microscopic analysis of EGFP penetration and distribution within arterial layers.

More Related Videos

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
08:11

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

Published on: August 25, 2017

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery
06:41

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery

Published on: January 31, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
08:43

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

Published on: November 30, 2012

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
08:11

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

Published on: August 25, 2017

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery
06:41

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery

Published on: January 31, 2017

Main Results:

  • 11R-EGFP demonstrated immediate and effective penetration into all layers of the rat basilar artery.
  • Transduction was particularly prominent in the tunica media.
  • This represents the first successful transduction of a PTD-fused protein into cerebral arteries.

Conclusions:

  • The 11R PTD offers a revolutionary approach for protein delivery to cerebral arteries.
  • This method holds significant potential for future therapeutic strategies targeting cerebral vasospasm.
  • Further research is needed to overcome existing challenges and optimize 11R applications for CV.