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Updated: Jun 25, 2026

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
Published on: June 14, 2016
Mass-mapping of ECM macromolecules by scanning transmission electron microscopy
Michael J Sherratt1, Helen K Graham, Cay M Kielty
1The University of Manchester, Manchester, M13 9PT, UK. michael.j.sherratt@manchester.ac.uk
Abstract:
In the scanning transmission electron microscope, the degree of electron scattering induced by biological specimens, such as ECM macromolecules, is dependent on the molecular mass. By calibrating the ratio of scattered to non-scattered electrons against a known mass standard, such as tobacco mosaic virus, it is possible to quantify absolute changes in both mass and mass distribution. These mass mapping approaches can provide important information on ECM assembly, organisation, and interactions which is not obtainable by other means.
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