Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Tissue engineering and cell-populated collagen matrices.

Paul D Kemp1

  • 1Intercytex Ltd, Innovation House, Manchester, UK. pkemp@intercytex.com

Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment of burn contractures with allogeneic human dermal fibroblasts improves Vancouver scar scale: A phase I/II trial.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2021
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Tissue engineering constructs living tissues for clinical use. Collagen lattices are a key method, supporting cell growth and enabling studies on cell-matrix interactions, particularly tension

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Tissue engineering aims to create functional 3D cellular constructs for tissue replacement and studying complex cell interactions.
  • In vitro 3D cell organization requires a provisional extracellular matrix (ECM) scaffold.
  • Current methods involve culturing cells on synthetic meshes or embedding them within lattices like type I collagen.

Purpose of the Study:

  • To explore the use of collagen lattices as a provisional ECM for tissue engineering applications.
  • To investigate the utility of this model system for studying cell-matrix interactions, specifically the effect of tension on cell phenotype.

Main Methods:

  • Embedding stromal cells within a 3D type I collagen lattice.
  • Utilizing the contracted collagen lattice for applications such as supporting epithelial growth for skin replacement.

More Related Videos

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Related Experiment Videos

Last Updated: Jun 25, 2026

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

  • Employing the model system to conduct experiments on cell-matrix interactions under tension.

    • Main Results:

    • Collagen lattices effectively support epithelial growth and differentiation, leading to skin replacement applications.
    • Successful clinical treatment of chronic ulcers using this collagen lattice model.
    • Demonstrated utility of the model for studying how mechanical tension influences cell phenotype.

    Conclusions:

    • 3D collagen lattices are a viable provisional ECM in tissue engineering.
    • This model system has proven effective for both clinical applications (e.g., skin regeneration) and fundamental research into cell-matrix dynamics.
    • Further research can leverage this system to understand mechanobiology in engineered tissues.