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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Updated: Jun 25, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Frontiers in fluorescence microscopy.

José Rino1, José Braga, Ricardo Henriques

  • 1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal. joserino@fm.ul.pt

The International Journal of Developmental Biology
|February 28, 2009
PubMed
Summary
This summary is machine-generated.

Novel imaging techniques have transformed biology, allowing scientists to observe living cells and organisms in unprecedented detail. This shift moves beyond traditional methods relying on fixed specimens, offering new insights into cellular function.

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Area of Science:

  • Cell biology
  • Microscopy
  • Biotechnology

Background:

  • Historically, biological studies relied on fixed, dehydrated, and stained specimens.
  • This limitation hindered the understanding of dynamic cellular processes in living organisms.
  • Over 150 years of research were constrained by these indirect observation methods.

Purpose of the Study:

  • To highlight the revolution in biological imaging.
  • To emphasize the shift from static to dynamic observation of life.
  • To underscore the impact of new technologies on understanding cellular function.

Main Methods:

  • Development of novel imaging techniques.
  • Advancements in microscopy and live-cell imaging.
  • Application of these techniques in biological research.

Main Results:

  • Scientists can now observe the structures of life in real-time.
  • Detailed visualization of living cells and organisms is now possible.
  • A deeper understanding of cellular functions is being achieved.

Conclusions:

  • Modern imaging technologies have fundamentally changed biological research.
  • The ability to study live specimens offers unparalleled insights.
  • This technological leap continues to drive discoveries in life sciences.