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Related Experiment Video

Updated: Jun 5, 2026

Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

Published on: January 17, 2020

Protocol for generating human iPSC-derived cardiomyocytes using VCAM1-based sorting and micropatterned culture.

Marta Furtado¹, Marta Ribeiro², Teresa Carvalho³

¹Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, Lisboa 1649-028, Portugal; Gulbenkian Institute for Molecular Medicine, Avenida Professor Egas Moniz, Lisboa 1649-028, Portugal.

|June 3, 2026

Summary

This summary is machine-generated.

This study presents a new protocol for generating human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Combining fluorescence-activated cell sorting and micropatterned substrates improves iPSC-CM generation for research.

Keywords:

Cell Differentiation Developmental biology Organoids Stem Cells

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Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

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Published on: September 27, 2018

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Last Updated: Jun 5, 2026

Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

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Published on: January 17, 2020

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Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Published on: September 27, 2018

Area of Science:

Cardiovascular Biology
Stem Cell Biology
Biotechnology

Background:

Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are crucial for disease modeling and regenerative medicine.
Current iPSC-CM generation methods face challenges like variability, immaturity, and heterogeneity.
Addressing these limitations is key to advancing cardiac research and therapies.

Purpose of the Study:

To develop an optimized protocol for generating high-quality human iPSC-CMs.
To improve iPSC-CM maturation and reduce population heterogeneity.
To provide a reproducible method for cardiac cell generation.

Main Methods:

Cardiac differentiation of human induced pluripotent stem cells (iPSCs) in 3D culture.
Enrichment of iPSC-CMs using fluorescence-activated cell sorting (FACS) for VCAM1-positive cells.
Maturation of iPSC-CMs using 2D micropatterned substrate culture.

Main Results:

The combined FACS and micropatterning approach yields enriched populations of iPSC-CMs.
This protocol addresses inter-differentiation variability and cell immaturity.
Micropatterned culture promotes enhanced maturation of iPSC-CMs.

Conclusions:

This protocol offers a robust method for generating mature and homogeneous iPSC-CMs.
The technique enhances the utility of iPSC-CMs for disease modeling and therapeutic applications.
Further research can leverage this protocol for advancing cardiovascular regenerative medicine.