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Updated: Jun 25, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Mobile elements of an Azospirillum brasilense Sp245 85-MDa plasmid involved in replicon fusions
Elena I Katsy1, Alexei G Prilipov
1Laboratory of Microbial Genetics, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia. katsy@ibppm.sgu.ru
Abstract:
Sequence analysis of approximately 25kb of an Azospirillum brasilense Sp245 85-MDa ( approximately 142kb) plasmid, p85, identified two novel IS elements mediating p85 fusions with a suicide plasmid vector, pJFF350. These IS elements, 1465-bp ISAzba1 and 1112-bp ISAzba3, belong to the IS256 family and to the IS5 family/IS903 group, respectively. Truncated ISAzba2 from the ISL3 family was found near one of the copies of ISAzba1 that flank pJFF350 in p85::pJFF350. As another factor potentially contributing to the known genetic plasticity of p85, a phage integrase gene was identified in this plasmid.
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