Related Experiment Video
Updated: Jun 25, 2026
11:42
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
Published on: October 27, 2017
Exploring differential gene expression in zebrafish to teach basic molecular biology skills
Angela Schmoldt1, Jennifer Forecki, Dena R Hammond
1Department of Biological Sciences, University of Wisconsin-Milwaukee, 600 E. Greenfield Ave., Milwaukee, WI 53204, USA.
Zebrafish
|March 3, 2009
Summary
This molecular biology course used whole-mount zebrafish embryos and in situ hybridization to teach differential gene regulation. Students gained research experience and learned essential molecular biology techniques.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- Engaging students in original research is crucial for scientific education.
- Traditional courses often lack hands-on molecular biology experience.
- Zebrafish embryos offer a powerful model for studying gene regulation.
Purpose of the Study:
- To design and implement an intensive workshop course for undergraduates and graduate students.
- To teach basic molecular biology skills using in situ hybridization.
- To explore differential gene regulation in whole-mount zebrafish embryos.
Main Methods:
- A 13-day intensive workshop format was employed.
- In situ hybridization techniques were used on whole-mount zebrafish embryos.
- The course focused on an ongoing research project within the laboratory.
Main Results:
- Students were successfully exposed to various molecular biology techniques.
- The course provided a practical context for understanding differential gene regulation.
- Both students and instructors reported beneficial learning outcomes.
Conclusions:
- The workshop successfully integrated original research with molecular biology skill development.
- In situ hybridization in zebrafish embryos is an effective tool for teaching gene regulation.
- This course format offers a valuable model for science education.
