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Updated: Jun 25, 2026
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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
High-level production of a humanized immunoRNase fusion protein from stably transfected myeloma cells
Jürgen Krauss1, Evelyn Exner, Athanasios Mavratzas
1National Center for Tumor Diseases, University of Heidelberg, Heidelberg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2009
Abstract:
ImmunoRNases represent a highly attractive alternative to conventional immunotoxins for cancer therapy. Quantitative production of immunoRNases in appropriate expression systems, however, remains a major challenge for further clinical development of these novel compounds. Here we describe a method for high-level production and purification of a fully functional immunoRNase fusion protein from supernatants of stably transfected mammalian cells.
