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Related Experiment Video

Updated: Jun 25, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Decomplexing biofluids using microchip based acoustophoresis.

Per Augustsson1, Jonas Persson, Simon Ekström

  • 1Department of Electrical Measurements, Division of Nanobiotechnology, Lund University, P.O. Box 118, S-221 00, Lund, Sweden. per.augustsson@elmat.lth.se

Lab on a Chip
|March 4, 2009
PubMed
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This summary is machine-generated.

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Acoustophoresis in continuous flow enables efficient microbead washing and analyte extraction, from peptides to viruses. This acoustic separation technique minimizes carry-over, ideal for isolating rare species in complex samples.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biophysics

Background:

  • Efficient separation and washing of microbeads are crucial for complex biological sample analysis.
  • Existing methods can be time-consuming and may lead to analyte loss or contamination.

Purpose of the Study:

  • To develop and demonstrate a highly efficient, microscaled, continuous flow washing and extraction method for microbeads using acoustophoresis.
  • To showcase the application of this technique for isolating specific analytes, including peptides and viruses, from complex mixtures.

Main Methods:

  • Utilized acoustophoresis, leveraging acoustic radiation force in an ultrasonic standing wave and laminar flow.
  • Employed functionalized microbeads for analyte capture and acoustic manipulation for fluid translation.

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Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells
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Last Updated: Jun 25, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
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Published on: November 23, 2015

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
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  • Demonstrated selective extraction using metal oxide affinity capture (MOAC) beads for phosphopeptides and specific binders for phage capture.

    • Main Results:

    • Achieved highly efficient washing and extraction of microbeads for diverse analytes (peptides to viruses).
    • Demonstrated selective phosphopeptide extraction with minimal carry-over (1-50 ppm).
    • Showcased exceptional phage capture efficiency with a cross-contamination level of 10^-6.

    Conclusions:

    • Acoustophoresis provides a robust and efficient method for microbead washing and analyte extraction in a continuous flow format.
    • The technique is suitable for isolating rare species from complex biological matrices.
    • Offers flexibility for sample volume and seamless integration into automated analytical workflows.