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Updated: Jun 25, 2026

A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

Published on: September 13, 2018

Activated spinal cord ependymal stem cells rescue neurological function.

Victoria Moreno-Manzano¹, Francisco Javier Rodríguez-Jiménez, Mireia García-Roselló

¹Cellular Reprogramming Laboratory, Valencia, Spain.

Stem Cells (Dayton, Ohio)

|March 5, 2009

Summary

This summary is machine-generated.

Modulating endogenous ependymal stem/progenitor cells (epSPCs) after spinal cord injury (SCI) enhances their proliferation and self-renewal. Transplantation of these cells promotes significant motor recovery in SCI models.

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Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

Published on: December 17, 2014

Area of Science:

Neuroscience
Regenerative Medicine
Stem Cell Biology

Background:

Spinal cord injury (SCI) leads to paralysis with no effective restorative therapies.
Adult spinal cords contain ependymal stem/progenitor cells (epSPCs) with potential for endogenous repair.
epSPCs are influenced by immune responses following SCI.

Purpose of the Study:

To investigate the potential of endogenous epSPCs for spinal cord repair.
To characterize epSPCs derived from SCI models (epSPCi).
To evaluate the therapeutic efficacy of epSPCi transplantation in a rat SCI model.

Main Methods:

Culturing and characterizing epSPCs from SCI rats (epSPCi) versus control animals.
Analyzing genetic profiles and signaling pathways (e.g., Jak/Stat, MAPK) in epSPCi.
Differentiating epSPCs and epSPCi into oligodendrocytes and motoneurons.
Transplanting undifferentiated epSPCi or derived oligodendrocyte precursor cells into a rat SCI contusion model.

Main Results:

epSPCi exhibited a 10-fold increase in proliferation and enhanced self-renewal compared to control epSPCs.
Inflammation significantly influenced signaling pathways in epSPCi.
epSPCi cultures yielded a higher number of differentiated oligodendrocytes and motoneurons.
Transplanted epSPCi cells migrated extensively and promoted significant motor function recovery within one week post-injury.

Conclusions:

Modulating endogenous epSPCs is a promising cell-based strategy for spinal cord repair.
epSPCi demonstrate enhanced proliferative and differentiation capacities.
Transplantation of epSPCi leads to functional recovery in SCI models, highlighting their therapeutic potential.