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Updated: Jun 25, 2026
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
Published on: March 8, 2022
[Preliminary study on molecular mechanism of curcumine anti-mouse melanoma]
Fei Gui1, Wei-Feng Ma, Shao-Hui Cai
1Pharmacology Laboratory Center, Jinan University, Guangzhou 510632, China.
Objective:
To investigate the effects of curcumine on mouse B16 melanoma growth and possible mechanism of Bcl-2, P53 and glutathione in tumor cells.
Methods:
The inhibitory effect on growth of melanoma in vivo were examined by mice melanoma models transplanted B16 cells to C57BL/6J mice. MTT method was used to assay the contribution of curcumine to B16 cells in vitro. The apoptosis and expression of Bcl-2, P53 gene of B16 cells were analyzed by flow cytometry, and HPLC assay was used to detect the change of GSH in B16 melanom tissues of C57BL/6J mouse caused by curcumine.
Results:
Curcumine had obvious inhibitory effect on the growth of mouse B16 melanoma in time and dose dependent manner and the gene expression of bcl-2 in B16 cells decreased after 24 hours supplied with curcumine, whereas P53 protein expression increased; Curcumine depressed the GSH quantity in melanoma tissues.
Conclusion:
The growth inhibitory effect of curcumine on mouse melanom is proved in vivo and in vitro respectively. Curcumine can induce some cells to apoptosis which may be relevant to downregulation of bcl-2 expression and upregulation of P53 expression as well as exhaustion of GSH in tumor organization.
Insights
Curcumin effectively inhibits B16 melanoma growth in mice and in vitro. This anticancer effect may stem from inducing apoptosis via Bcl-2 downregulation, P53 upregulation, and glutathione depletion.
Area of Science:
- Oncology
- Pharmacology
Context:
- Melanoma is a significant form of skin cancer.
- Curcumin, a compound from turmeric, shows potential anticancer properties.
Purpose:
- To evaluate curcumin's inhibitory effects on B16 melanoma growth.
- To elucidate the mechanisms involving Bcl-2, P53, and glutathione.
Summary:
- Curcumin demonstrated a dose- and time-dependent inhibition of B16 melanoma growth in vivo and in vitro.
- Curcumin treatment led to decreased Bcl-2 expression, increased P53 protein levels, and reduced glutathione (GSH) in tumor tissues, suggesting apoptosis induction.
Impact:
- Provides evidence for curcumin's therapeutic potential against melanoma.
- Highlights key molecular targets for curcumin's anticancer activity.
