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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

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Related Experiment Video

Updated: Jun 25, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

Mapping of RNA-protein interactions.

Subash Chandra Bose Gopinath1

  • 1Institute for Biological Resources and Functions & Center for Applied Near Field Optics Research (CAN-FOR), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba City 305-8562, Ibaraki, Japan.

Analytica Chimica Acta
|March 7, 2009
PubMed
Summary

Mapping strategies are crucial for understanding RNA-protein interactions, which are vital for cellular functions. This overview explores methods to map these essential molecular complexes, aiding in the study of functional RNAs and RNA-aptamers.

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Last Updated: Jun 25, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

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Published on: May 24, 2017

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

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Published on: September 30, 2016

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • RNA-protein interactions are fundamental to numerous biological processes across all life forms.
  • Diverse RNA conformations are necessary for the selective recognition of proteins.
  • Existing quantitative analyses of RNA-protein complexes lack specific interaction mapping.

Purpose of the Study:

  • To provide an overview of mapping strategies for RNA-protein interactions.
  • To discuss the interactions between functional RNAs, RNA-aptamers, and their target proteins.
  • To highlight the importance of analytical mapping for probing specific RNA-protein contacts.

Main Methods:

  • Review of current analytical procedures for RNA-protein complex analysis.
  • Focus on mapping strategies to identify specific interaction sites.
  • Discussion of methods applicable to functional RNAs and RNA-aptamers.

Main Results:

  • Analytical-based mapping is essential for detailed understanding of RNA-protein interactions.
  • Mapping strategies reveal specific contacts crucial for selective protein recognition.
  • The overview synthesizes knowledge on mapping techniques for diverse RNA-protein systems.

Conclusions:

  • Mapping strategies are indispensable tools for dissecting RNA-protein interactions.
  • Understanding these interactions through mapping is key to elucidating their biological functions.
  • This review consolidates approaches for mapping functional RNAs and RNA-aptamer interactions.