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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Published on: March 7, 2018

Prostate specific antigen detection using microgapped electrode array immunosensor with enzymatic silver deposition.

Yong Huang1, Tai-Hong Wang, Jian-Hui Jiang

  • 1State Key Laboratory for Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.

Clinical Chemistry
|March 7, 2009
PubMed
Summary
This summary is machine-generated.

A novel electrical immunosensor accurately detects prostate specific antigen (PSA) at very low concentrations. This sensitive biosensor offers a promising tool for early disease diagnosis in biomedical research.

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Published on: February 23, 2020

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Immunochemistry

Background:

  • Trace protein analysis is crucial for biomedical research and clinical diagnosis.
  • Detecting proteins at low concentrations presents a significant challenge in immunochemistry.
  • An electrical immunosensor was developed for prostate specific antigen (PSA) detection.

Purpose of the Study:

  • To develop and validate a novel electrical immunosensor for sensitive PSA detection.
  • To assess the performance of the immunosensor in quantifying PSA in human serum samples.
  • To establish the potential of this technology for broader molecular detection applications.

Main Methods:

  • Utilized a microgapped interdigitated electrode array (MGIDEA) biosensor.
  • Employed enzymatic silver deposition to create electrical connectivity across microgaps.
  • Quantified PSA concentration by measuring changes in electrical conductance.

Main Results:

  • Demonstrated a linear response to PSA concentrations across a 6-decade range (1.0 pg/L to 1.0 µg/L).
  • Achieved a low limit of detection of 0.9 pg/L for PSA.
  • Showed agreement within 10% with a commercial chemiluminescent immunoassay for PSA in patient serum.

Conclusions:

  • The MGIDEA-based electrical immunosensor is highly sensitive and specific.
  • The method exhibits a low background signal and a low limit of detection.
  • This technology holds significant potential for molecular detection in diverse biomedical fields.