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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 25, 2026

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
10:57

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

Gene targeting in mouse embryonic stem cells.

Lino Tessarollo1, Mary Ellen Palko, Keiko Akagi

  • 1Mouse Cancer Genetics Program, NCI-Frederick, Frederick, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2009
PubMed
Summary
This summary is machine-generated.

Creating precise mouse models for research requires careful genetic engineering. This guide details advanced techniques like cre/loxP and recombineering for sophisticated genome manipulation in mice.

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Mouse Genome Engineering Using Designer Nucleases

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Related Experiment Videos

Last Updated: Jun 25, 2026

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
10:57

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
12:13

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Area of Science:

  • * Genetics and Genomics
  • * Animal Models in Research

Background:

  • * The scientific utility of genetically modified mouse models hinges on precise engineering of targeted mutations.
  • * Previous limitations in mouse genome alteration included a lack of conditional targeting technologies and reliance on conventional cloning methods.
  • * Advancements in genetic engineering technologies have significantly improved the ability to manipulate the mouse genome.

Purpose of the Study:

  • * To describe the process of engineering targeted mutations for generating mouse models.
  • * To provide a practical tutorial for utilizing bioinformatics tools in genetic locus targeting.
  • * To address practical considerations for successful genetic locus manipulation.

Main Methods:

  • * Utilized cre/loxP and recombineering technologies for advanced mouse genome manipulation.
  • * Employed publicly available bioinformatics tools for processing genetic information.
  • * Developed a step-by-step process for generating a targeting vector for mouse models.

Main Results:

  • * Demonstrated a refined methodology for engineering targeted mutations in mice.
  • * Provided a practical guide for researchers to generate specific mouse models.
  • * Highlighted the importance of careful engineering in creating scientifically valuable mouse models.

Conclusions:

  • * Advanced technologies like cre/loxP and recombineering have overcome previous limitations in mouse genome engineering.
  • * Careful consideration of practical aspects is crucial for successfully targeting specific genetic loci.
  • * The described process and tutorial facilitate the sophisticated manipulation of the mouse genome for research purposes.