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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

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Published on: September 16, 2012

Systemic first-line phenotyping.

Valérie Gailus-Durner1, Helmut Fuchs, Thure Adler

  • 1Institute of Experimental Genetics, Helmholtz Zentrum München, Neuherberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2009
PubMed
Summary
This summary is machine-generated.

The International Mouse Knockout Consortium will create thousands of mouse disease models. Mouse clinics are essential for characterizing and disseminating these models to researchers.

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Area of Science:

  • Genomics
  • Translational Medicine
  • Animal Models

Background:

  • Completion of the mouse genome sequence necessitates functional analysis of all mammalian genes.
  • The International Mouse Knockout Consortium aims to generate over 30,000 gene-targeted mutations in ES cells, creating numerous mouse disease models.
  • Effective characterization, archiving, and dissemination are crucial for maximizing the value of these mouse models.

Purpose of the Study:

  • To outline the organization and function of mouse phenotyping centers (mouse clinics).
  • To present selected methods employed in first-line phenotyping of mouse models.
  • To highlight the role of mouse clinics in supporting large-scale systemic phenotyping initiatives.

Main Methods:

  • Systemic first-line phenotyping using standardized protocols.
  • Comprehensive data collection and archiving.
  • Dissemination of characterized mouse models to the research community.

Main Results:

  • Mouse clinics provide essential infrastructure and expertise for large-scale phenotyping.
  • Standardized phenotyping enables efficient characterization of thousands of mouse models.
  • Facilitated access to diverse mouse models accelerates biomedical research.

Conclusions:

  • Mouse clinics are vital for realizing the full potential of the International Mouse Knockout Consortium's efforts.
  • Systematic phenotyping and data sharing are critical for advancing functional genomics and disease modeling.
  • The German Mouse Clinic serves as a model for organizing and operating effective phenotyping centers.