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Related Experiment Video

Updated: May 10, 2026

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
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Published on: January 12, 2018

Lentiviral transgenesis.

Alexander Pfeifer1, Andreas Hofmann

  • 1Institut für Pharmakologie und Toxikologie, Universität Bonn, , Bonn, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2009
PubMed
Summary
This summary is machine-generated.

Lentiviral vectors enable efficient gene transfer into various cells, including oocytes and embryos, for creating transgenic animals. This lentiviral transgenesis technology offers a versatile approach for stable transgene expression across diverse species.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Lentiviral vectors facilitate efficient gene delivery into diverse cell types, including stem cells and non-dividing cells.
  • Lentiviruses integrate their genome into host chromosomes, enabling stable transgene expression and potential latency.

Purpose of the Study:

  • To explore the practical applications of lentiviral vectors for generating transgenic animals.
  • To detail methods for lentiviral vector preparation and gene delivery into early embryos.
  • To discuss the use of lentiviral RNA interference.

Main Methods:

  • Gene delivery using lentiviral vectors into oocytes and early embryos.
  • Vector preparation protocols for lentiviral transgenesis.
  • Application of lentiviral RNA interference techniques.

Main Results:

  • Lentiviral transgenesis has demonstrated high efficiency in various species, including mice, rats, pigs, cattle, and birds.
  • Stable transgene expression achieved through lentiviral integration into the host genome.
  • Successful gene transfer into oocytes and early embryos for animal transgenesis.

Conclusions:

  • Lentiviral transgenesis is a highly efficient and versatile technology for generating transgenic animals.
  • The method holds significant potential for widespread adoption in animal biotechnology.
  • Further research into practical aspects like vector preparation and RNA interference will enhance its utility.