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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Transposon mutagenesis in mice.

David A Largaespada¹

¹University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 7, 2009

Summary

This summary is machine-generated.

Creating mutant mouse strains is crucial for understanding mammalian genome function. The Sleeping Beauty transposon system offers a powerful tool for generating these mutations efficiently.

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Area of Science:

Genomics and Molecular Biology
Mammalian Genetics

Background:

Understanding the mammalian genome's functional landscape is a key challenge in biomedical research.
Genome projects and expression analyses raise questions about gene quantity, type, and functional elements.

Purpose of the Study:

To review tools and methods for creating mutant mice using in vivo, germline transposition.
To explore the potential of transposons, specifically the Sleeping Beauty system, for genetic screens and regional saturation mutagenesis.

Main Methods:

Utilizing transposon-based systems for generating mutations in mouse genes.
Employing the Sleeping Beauty (SB) transposon for its local hopping capability.

Main Results:

Transposons are valuable tools for creating comprehensive mutant mouse strain resources.
The Sleeping Beauty transposon system facilitates in vivo, germline transposition for mutagenesis.

Conclusions:

Transposon-mediated mutagenesis, particularly with the Sleeping Beauty system, is a promising approach for advancing functional genomics in mice.
This technology supports initiatives for large-scale mutagenesis and forward genetic screens.